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Czapek's Agar (CZA) |
Hay infusion agar |
Malt Extract Agar (MEA) |
Modified Dixon's agar (mDixon) |
Potato Dextrose Agar (PDA) |
Sabouraud agar or Sabouraud's dextrose agar (SDA) |
Czapek's agar is used for cultivation of fungi and certain other organisms.
The pH of the medium should be 7.3.
This medium is recommended for growing Aspergillus spp. Penicillium spp. and Paecilomyces spp. As well as some other fungi. Only organisms that can use nitrate (NO3-) as a source of nitrogen can grow on this medium.
Updated: 2022-02-03.
ContentsHay infusion agar is a nutrient deficient medium, which can be used for subcultivation of fungi (for instance Actinomortierella wolfii), to get them to sporulate.
The final pH of the medium should be 6.2.
Updated: 2021-01-14.
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Malt extract agar (MEA) is used for cultivation of yeasts and moulds.
The pH of the medium should be 4.7.
The high concentration of maltose makes this medium particularly useful for cultivation of yeasts and moulds.
Updated: 2022-02-03.
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Dixon's agar is mainly used for growing Malassezia furfur, but a modified variant (mDixon) can also be used for growing other dermatophytes.
The pH of the medium should be 6.0.
Most Malassezia species (except M. pachydermatis) are lipophilic i.e. they require the addition of lipids to the culture medium. Other dermatophytes can also be grown in mDixon.
Updated: 2021-09-16.
ContentsMalt extract agar is used for cultivation of yeasts and moulds.
The pH of the medium should be 5.6.
The medium can be supplementet with acid and/or antibiotics to inhibit bacterial growth.
Updated: 2022-02-03.
ContentsImage A shows a plate containing Sabouraud Agar before cultivation. Image B shows the same kind of plate on which Hanseniaspora uvarum has been cultivated at 30°C during 5 days. - Click on the image to enlarge it (opens in a new window/tab).
Image: Ingrid Hansson (BVF,SLU), Lise-Lotte Fernström (BVF, SLU) and Karl-Erik Johansson (BVF, SLU).
Sabouraud agar is mainly used to grow dermatophytes and other types of yeasts and molds. Sabaroudagar can also be used to grow filamentous bacteria, e.g. species within the genus Nocardia.
The pH of the medium should be 5.6 and this relatively low pH inhibits the growth of many bacteria.
This medium is not always recommended for primary isolation, as it is not nutritious enough for some fastidious fungi. This is especially true for many of the dimorphic fungi.
Updated: 2021-05-14.
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